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. 2011 Jul 29;286(39):33819–33831. doi: 10.1074/jbc.M111.269779

FIGURE 2.

FIGURE 2.

Negative ion ESI-MS of lipid extracts from E. coli BLR(DE3)pLysS/pET15b and BLR(DE3)pLysS/pAt1g78690p. A, negative ion ESI-MS from m/z 500 to 1100 of lipid extract prepared from BLR(DE3)pLysS/pET15b. B, negative ion ESI-MS from m/z 500 to 1100 of lipid extract prepared from BLR(DE3)pLysS/pAt1g78690p. In A and B, the major ions in m/z 650–800 correspond to [M − 2H]2− ions of CL and [M − H]− ions of PE and PG. C, negative ion ESI-MS from m/z 900 to 1050 of purified accumulating lipid. The major [M − H]− ions correspond to acyl-PG molecular species as shown in Table 1. D, negative ion MS/MS of m/z 983.7. The inset shows the major product ions from a predicted precursor ion. At the given molecular mass of 983.7, several distinct molecular species of acyl-PG are possible. The inset shows one possible molecular species consistent with the product ion spectra. The MS/MS technique we employed does not allow for definitive assignment of the acyl chains to the sn-1, sn-2, sn-2′, or sn-3′ position.