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Phospholipase D treatment of the lipid that accumulates upon overexpression of AT1G78690 in E. coli. Samples were treated with phospholipase D, displayed by TLC using solvent B, and visualized by exposure to iodine vapor. Lane 1, total lipid extracts from BLR(DE3)pLysS/pET15b; lane 2, purified accumulating lipid; lane 3, acyl-PG standard; lane 4, N-acyl-PE standard.
