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. 2011 Jul 29;286(39):33819–33831. doi: 10.1074/jbc.M111.269779

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Base hydrolysis of ³²P-labeled lipids from BLR(DE3)pLysS/pET15b and BLR(DE3)pLysS/pAt1g78690p. GPLs were in vivo labeled by culturing in the presence of [³²P]PO₄³⁻ and extracted as described under “Experimental Procedures.” 1000 dpm of each sample was treated with base, spotted to TLC, and developed in solvent A. As indicated, lipid extracts from BLR(DE3)pLysS/pAt1g78690p or BLR(DE3)pLysS/pET15b were treated or not treated with 0.2 m NaOH. The predicted products and the migration of the base hydrolysis products of acyl-PG and N-acyl-PE are shown. Base treatment of the standards yields similar products (supplemental Fig. 3).