FIGURE 5.

Acylation of ³²P-labeled 1-acyl-PE and 1-acyl-PG by At1g78690p. Crude extracts (0.05 mg/ml) prepared from induced BLR(DE3)pLysS/pET15b or BLR(DE3)pLysS/At1g78690p were tested for the ability to acylate ³²P-labeled 1-acyllyso-PE, or 1-acyllyso-PG using palmitoyl-CoA as the acyl donor in a 10-min reaction at 37 °C. The E. coli lysophospholipase L2, PldB (35–37), is known to catalyze the synthesis of acyl-PG from PG and lyso-PE or lyso-PG and may be responsible for the acyl-PG formed in these assays.