Skip to main content
. 2011 Jul 29;286(39):33819–33831. doi: 10.1074/jbc.M111.269779

FIGURE 8.

FIGURE 8.

Determination of the site of acylation of 1-acyllyso-PE. Solubilized membranes prepared from induced BLR(DE3)pLysS/pET15b or BLR(DE3)pLysS/At1g78690p were incubated in an in vitro assay mixture using 1-oleoyllyso-PE as the acyl acceptor and arachidonyl-CoA as the acyl donor as described under “Experimental Procedures.” The lipids from the reaction mixture were extracted and analyzed using LC/ESI-MS and MS/MS. A, negative ion LC/ESI-MS from m/z 660 to 780 of lipids extracted from the reaction of solubilized membranes from BLR(DE3)pLysS/pET15b. B, negative ion ESI-MS from m/z 660 to 780 of lipids extracted from the reaction of solubilized membranes from BLR(DE3)pLysS/pAt1g78690p. The mass spectra in A and B are of the material eluting between minutes 22.0 and 25.0 of the normal phase chromatography. C, negative ion MS/MS of the in vitro product at m/z 764.5. D, positive ion MS/MS of the in vitro product at m/z 766.5. E, positive ion MS/MS of 16:0, 18:1 PE standard. F, positive ion MS/MS of N-arachidonyl 18:1 lyso-PE. The insets show the major product ions predicted from each precursor ion.