FIGURE 3.

A conserved hydrophobic di-Leu-based motif in the N terminus of p63RhoGEF is not required for PM association or for SRE activity in cells. A, amino acid sequence alignment showing conservation of the di-Leu-based hydrophobic motif in human (Q86VW2), bovine (XP_002687606.1), and Xenopus (NP_001123746.1) orthologs. B, deletion of the ¹²¹LTLLTTLL¹²⁸ motif does not disrupt PM localization of EGFP-p63RhoGEF. Data shown are representative of four experiments. Scale bar, 10 μm. C, the Δ¹²¹LTLLTTLL¹²⁸ variant also retains wild-type basal activity in cells. The data shown are representative of five independent experiments performed in triplicate. Error bars, S.D.