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. 2011 Aug 11;286(39):33709–33718. doi: 10.1074/jbc.M110.193870

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Holo-TFIIB complex is susceptible to high ionic strength and sediments between TFIID and TFIIH complexes. A, affinity-purified HA-tagged TFIIB and rTFIIB were subjected to sedimentation analysis in 5–30% (v/v) glycerol gradient in 500 mm KCl. Fractions were collected and processed as in Fig. 2. B, affinity-purified preparations of TFIIB (panel i), TBP (panel ii), and Kin28 (panel iii) were subjected to sedimentation analysis in 5–30% (v/v) glycerol gradient in 150 mm KCl as described previously. Fractions were collected, processed as above, and probed for TFIIB and TBP or TFIIB and Kin28.