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. 2011 Aug 11;286(39):33709–33718. doi: 10.1074/jbc.M110.193870

FIGURE 4.

FIGURE 4.

Gene looping requires the CF1 complex and poly(A) polymerase. A, schematic representation of MET16 and INO1 indicating the positions of AluI restriction sites (vertical lines) and PCR primers (arrows) used in CCC analysis. B, CCC analysis of MET16 and INO1 to detect gene looping in W303-1a (wild type) and mutant strains of Rna14 (rna14-1), Pcf11 (pcf11-2), Hrp1 (hrp1-5), and poly(A) polymerase (pap1-1) following 120 min of induction followed by incubation at either permissive (25 °C) or non-permissive (37 °C) temperatures. P1T1 PCR reflects the looping signal, whereas Control PCR represents the loading control indicating that equal amount of template DNA was present in each of the CCC reactions. C, quantification of the CCC results shown in B, representing ChIP signal/Input signal. Error bars indicate one standard deviation.