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Induced transcription in the looping-defective mutant of TFIIB exhibits a kinetic lag. A and D, schematic depiction of MET16 and INO1 indicating the positions of RT-PCR primer pairs. B and E, RT-PCR analysis of MET16 and INO1 in WT and looping-defective sua7-1 strains following transfer of cells to transcription-inducing conditions at the indicated time points. C and F, quantification of the data shown in B and E. Error bars indicate one standard deviation.
