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. 2001 Apr 10;98(8):4764–4769. doi: 10.1073/pnas.071058398

Figure 2.

Figure 2

Retargeting of GFP-fusion HD fragments to the nucleus by anti-HD C4 sFv-HA-nls. Immunofluorescence of HD-Q103-myc-His6 with C4 sFv-HA-nls (b, d, f) or a negative control sFv-HA-nls intrabody (a, c, e), after 48-h cotransfection in COS-7. HD-Q103-myc-His6 was detected by 9E10 mAb, followed by FITC-labeled goat anti-mouse IgG antibodies (c, d). The intrabodies were detected with polyclonal anti-HA antibodies, followed by rhodamine-labeled goat anti-rabbit IgG antibodies (a, b). (e, f) Dual staining of the same fields.