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. 2001 Apr 10;98(8):4764–4769. doi: 10.1073/pnas.071058398

Figure 3.

Figure 3

Immunofluorescence of HD-polyQ-GFP in the presence of cotransfected anti-HD C4 sFv. (A) BHK-21 cells transfected with HD-polyQ-GFP (normal control, Q25; pathogenic, Q72 or Q103) alone or cotransfected with C4 intrabody or control (ML3–9 sFv-HA) at an sFv to antigen plasmid ratio of 5:1. Live cells are shown photographed at 48 h after transfection, showing aggregates as intense foci of fluorescence. Similar results were seen with HD-Q72-GFP cotransfections (not shown). (B) Double labeling of 5:1 C4/HD-polyQ-GFP cotransfections; HD-polyQ-GFP (green), C4 sFv (red, detected by anti-HA antibody and Alexa 568-conjugated secondary antibody) and merged image. Some aggregates are found in Q103 cotransfectants but never in Q25 transfectants; cells not expressing C4 intrabody (arrowheads) often display aggregates, and to a lesser degree, in some cotransfected cells (arrow).