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Substrate specificity of recombinant His-tagged non-mutated NeuTTM. The specificity was tested in the presence of 5 mm MgCl2 (pH 9.7) (A) or 1 mm MnCl2 (pH 10.4) (B). The incubation medium contained either 50 mm Na-CHES (pH 9.7) or 50 mm Na-CAPS (pH 10.4), 0.5 mm substrate, and 5 mm MgCl2 (pH 9.7) or 1 mm MnCl2 (pH 10.4). The temperature was 37 °C. Results are the mean ± S.D., n = 2–6. cPPPi, cyclic PPPi.
