FIGURE 3.

Kinetic properties of NeuTTM PPPase activity. The PPP_i concentration was 0.5 mm. A, dependence on temperature in the presence of 5 mm Mg²⁺ at pH 9.7 (○) or 0.8 mm Mn²⁺ at pH 10.4 (●) is shown. B, pH dependence in the presence of 10 mm Mg²⁺ (○) or 1 mm Mn²⁺ (●) at 37 °C is shown. C, dependence on substrate concentration at pH 7.1 (○) and 9.7 (●) at 37 °C is shown. The curves were drawn by non-linear regression of the Michaelis-Menten equation (n = 3). The buffers used for different pH values are given under “Experimental Procedures.” Results are the mean ± S.D.