FIGURE 3.

Protein expression in yeast sdh1–4Δ mutants. Immunoblot analyses were conducted as described under “Experimental Procedures” using 5 μg of cellular protein samples from yeast strains containing disruptions in each gene encoding a subunit of succinate dehydrogenase. Similar amounts of each extract were electrophoresed using two gels for immunoblot analyses using antisera for Cit2 or Act1. The level of Cit2 in each sample was compared with that of β-actin (Act1) using densitometry and normalized to the parental strain level.