FIGURE 4.

ESCRT-II and Vps20 nucleate a strand of Vps32 monomers that bind specifically to highly curved bilayers. A, left panel, representative AFM images of a bilayer assembled in the presence of ESCRT-II (100 nm), Vps20 (150 nm), and Vps32 (450 nm). Right panel, 4-fold magnification of the boxed region in the left panel. The arrow highlights the presence of a filament along the bilayer edge. A shade-height scale bar is shown to the right of each panel. White scale bar = 200 nm (left panel) and 100 nm (right panel). B, representative AFM images of an identical region of a bilayer formed in the presence of ESCRT-II (100 nm), Vps20 (150 nm), and Vps32 (450 nm) following the application of low force (left panel) and high force (right panel) with the AFM tip. A shade-height scale bar is shown to the right. White scale bar = 100 nm. C, the height relative to the bilayer is plotted along the lines shown in B. Color-coded arrowheads highlight the bilayer edges.