Skip to main content
. 2011 Aug 8;286(39):33879–33889. doi: 10.1074/jbc.M111.234997

FIGURE 4.

FIGURE 4.

CRAG knockdown and CRAG dominant negative mutant reduce polyQ- and MG132-induced c-Fos and AP-1 activities. A and B, effect of CRAG knockdown on polyQ-induced AP-1 (A) and c-Fos/c-Jun activations (B). C and D, effect of CRAG knockdown on MG132-induced AP-1 (C) and c-Fos/c-Jun activations (D). E and F, effect of expression of CRAGΔC (ΔC) on polyQ-induced AP-1 (E) and c-Fos/c-Jun activations (F). G and H, effect of expression of CRAGΔC on MG132-induced AP-1 (G) and c-Fos/c-Jun activations (H). Luciferase assay was performed with Neuro2A cells transfected with both pAP-1-Luc and pRL-CMV with indicated vector and/or siRNA (sc: scramble siRNA, siCRAG: CRAG-specific siRNA). For MG132 treatment, Neuro2A cells were treated with either DMSO or 10 μm MG132 for 24 h. Lysates of Neuro2A cells transfected with the indicated vector and/or siRNA were immunoblotted with indicated antibodies. The protein levels of c-Fos and c-Jun normalized with tubulin are shown in the right panel when the control value was arbitrarily set at 1.0. Error bars indicate ±S.D. (n = 4). *, p < 0.05; **, p < 0.01; ***, p < 0.005 (Student's t test).