FIGURE 5.

Expression of c-Fos partially rescued from polyQ- and MG132-induced cytotoxicity enhanced by CRAG knockdown. Effect of c-Fos on cytotoxicity enhanced by CRAG knockdown in cells expressing polyQ (A and B) and with MG132 treatment (C and D). Lysates of Neuro2A cells transfected with the indicated vector(s) were immunoblotted with the indicated antibodies. The protein levels of cleaved caspase3 normalized are shown in the upper panel when the control value was arbitrarily set at 1.0 (A and C). For MG132 treatment, Neuro2A cells were treated with either DMSO or 10 μm MG132 for 24 h. An ATP reduction assay was performed on Neuro2A cells as described above (B and D). Error bars indicate ±S.D. (n = 5). *, p < 0.05; ***, p < 0.005 (Student's t test).