FIGURE 2.

Correlation of the AT8 signal in the P1 fraction with the AT8 signal in the Sarkosyl-insoluble fraction. Four JNPL3 mouse brain samples with different degrees of Tau pathology were processed to generate the P1 fraction, and then these P1 samples were subjected to Sarkosyl extraction (11) (see Fig. 1). Both the P1 samples and the Sarkosyl-extracted samples were subjected to AT8 ELISA, so that for each brain there is a P1 ELISA read (x axis) and then a second read after Sarkosyl ELISA extraction (y axis). Whereas the absolute AT8 signal is somewhat reduced after Sarkosyl extraction, the correlation is almost perfect, indicating that our P1 preparation is adequate.