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. 2011 Aug 12;286(39):34457–34467. doi: 10.1074/jbc.M111.229633

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — AT8 ELISA. A, standard curve using AD and control brain homogenates. The normal Tau present in control brain does not lead to a detectable signal. B, AT8 Western blot and ELISA results in the P1 fraction of all mice in the study show a strong correlation (r² = 0.73) between the two measures. C, spiking experiment. Addition of PHF1 and MC1 at the indicated concentrations to JNPL3 P1 extracts does not interfere with signal detection by the AT8 ELISA, demonstrating that AT8 signal changes in brains of mice treated with these antibodies are not ELISA artifacts.