TABLE 3.

Kinetic data for caspase-2 mutants versus Ac-VDVAD-AFC

	Km		kcat		kcat/Km
	μm	Relative	s−1	Relative	1/s μm	Relative
Wild type	25	1.00a	0.60	1.00a	0.024	1.00a
T380A	220	8.60	0.130	0.22	0.00060	0.025
Y420A	84	3.30	0.520	0.87	0.0062	0.26
T380A/Y420A	No activityb

^a Relative activity defined by Ac-VDVAD-AFC cleaved by wild type caspase-2. Results are average of n = 3.

^b Tested up to 400 μm substrate.