FIGURE 3.

The interaction of oligomeric Aβ₄₂ with different proteins followed by ELISA. A, the plate was coated with 0.5 μg/well TPPP/p25 (▴), tubulin (○), or α-synuclein (●), then it was incubated with Aβ₄₂ at different concentrations. B, the apparent dissociation constants (K_d) characteristic for the interactions were evaluated by non-linear fitting of the hyperbolic saturation curves using the Microcal Origin 6.0 software. C, ○, the plate was coated with TPPP/p25 then incubated with Aβ₄₂. After incubation, tubulin was added in constant concentration (100 nm). After further incubation, anti-tubulin was added. ●, the plate was coated with TPPP/p25 and incubated with Aβ₄₂, 100 nm α-synuclein was added, and then anti-α-synuclein was added. The effect of the Aβ₄₂ was calculated as the absorbance at a given Aβ₄₂ concentration divided by the absorbance without Aβ₄₂. D, ○, the plate was coated with α-synuclein, then incubated with Aβ₄₂. After incubation, tubulin was added in constant concentration (100 nm). After further incubation, anti-tubulin was added. The average of three-five independent experiments and the S.E. is shown.