FIGURE 4.

Mapping of possible binding sites between Aβ₄₂ or α-synuclein and TPPP/p25 by MULTIPIN peptide technology (Pepscan analysis). The amino acid sequence of the TPPP/p25 was used to synthesize a complete set of overlapping decapeptides covalently attached to the surfaces of derivative polyethylene pins in a format compatible with standard ELISA. These overlapping peptides covered the entire sequence of the protein. Pins were coated with 4 μm Aβ₄₂ (A) or 1.5 μm α-synuclein (B), then anti-Aβ or anti-α-synuclein was added, respectively. The absorbances of the peptides (indicated by letters of the first amino acid of the decapeptides) are shown. The reaction of a pin-coupled peptide was scored positive (significance level, indicated by a line) when the ELISA absorption was significantly higher than the 2-fold average absorption of the peptides.