FIGURE 8.
Representative pictures of intracellular Aβ and TPPP/p25 aggregation in CHO7PA2 and CHO10 cells. Amyloid expressing CHO7PA2 cells (A–D) and TPPP/p25 expressing CHO10 cells (E–H) were transiently transfected with human recombinant TPPP/p25 and Aβ42, respectively, and immunostained for TPPP/p25 (C and G, gray), Aβ (B and F, red), and the autophagic marker LC3 (A, green), whereas the general intracellular membrane marker (BODIPY 500/510) was detected by its own signal (E, green). Note the appearance of cytoplasmic aggregates in the case of TPPP/p25 and β-amyloid co-expression on the merged pictures (D and H) that show no co-localization with either vacuole markers. Blue, DAPI. Scale bar, 10 μm.