TABLE 3.
Contributions of individual mutations to the splicing efficiency at the insertion site 2 in the KanR protein
| Mutant inteins | Mutationsa | Splicing efficiency | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| % | ||||||||||
| WT | 0 | |||||||||
| WT-1 | A(−1)G | 7 | ||||||||
| M3 | D24G | I58T | 0 | |||||||
| M3-3 | A(−)G | D24G | I58T | 65 | ||||||
| M30 | A(−)G | D24G | I58T | S18P | S107A | S122P | H143R | 98 | ||
| M30-6 | S18P | 0 | ||||||||
| M30-7 | S107A | 0 | ||||||||
| M30-8 | S122P | 0 | ||||||||
| M30-9 | H143R | 0 | ||||||||
| M30-1 | D24G | I58T | S18P | S107A | S122P | H143R | 71 | |||
| M30-2 | D24G | I58T | S18P | 22 | ||||||
| M30-3 | D24G | I58T | H143R | 38 | ||||||
| M30-4 | S18P | S107A | S122P | H143R | 15 | |||||
| M30-5 | S107A | S122P | H143R | 6 | ||||||
| M86 | D24G | I58T | S18P | S107A | S122P | H143R | S114P | P142L | 93 | |
| M86-1 | S18P | S107A | S122P | H143R | S114P | P142L | 11 | |||
| M86-2 | S107A | S122P | H143R | S114P | P142L | 20 | ||||
| M86-3 | D24G | I58T | H143R | P142L | 0 | |||||
| M86-4 | D24G | I58T | S107A | S114P | 0 | |||||
| M86-5 | D24G | I58T | S107A | 0 | ||||||
| M86-6 | D24G | I58T | S114P | 0 | ||||||
a The A(−1)G mutation is in the KanR sequence at the position immediately before the intein; all other mutations are relative to the wild type sequence of the Ssp DnaB mini-intein.