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Primers used for PCR
| Forward primer (5′-3′) | Reverse primer (5′-3′) | |
|---|---|---|
| TonB-A | GCATCCGGATCCGCATTCGACGCAAAATTTGGT | ATCTTACTCGAGATAAGAGTCAGTTTCAATGTT |
| TonB-B | GCATCCGGATCCGGTGACTTAGGTTATGCTGTT | ATCTTACTCGAGGAATTTCACTTTGTATCCTGC |
| TonB-B (Y800F) | TTGATTGCTAAATTCATTGATTCCTAT | ATAGGAATCAATGAATTTAGCAATCAA |
| TonB-B (D803A) | GCTAAATACATTGCCTCCTATGATGGT | ACCATCATAGGAGGCAATGTATTTAGC |
