FIGURE 2.

Az1 stimulates ODC but not cyclin D1, Aurora-A, and ΔNp73 degradation in vivo in transfected NIH3T3 cells. Constructs encoding Az1 and ODC were transfected into HEK-293T cells together with a construct encoding Aurora-A (A), cyclin D1 (B), or ΔNp73 (C). Cycloheximide (CHX) was added to the growth medium 24 h post-transfection, the cells were harvested at the indicated times, and the levels of the individual tested proteins were determined by Western blot analysis using anti-FLAG antibodies. Anti-actin antibodies were used to normalize the amount of protein loaded in each lane. The experiments presented were repeated three times, yielding practically identical results. Deg, degradation.