FIGURE 4.

Rod PDE6 expressed in Nrl^−/−cpfl1 mice is functionally active. A, immunoprecipitation (IP) of assembled rod PDE6 αβγ subunits from retinal extracts of Nrl^−/− and Nrl^−/− cpfl1 mice at P30 using ROS-I monoclonal antibody. After ROS-I IP, immunoblots were probed with rod or cone-specific PDE6 antibodies as indicated. Control IP with nonspecific mouse IgG is shown. B, amount of total cGMP, measured in dark (DA) and light-adapted (LA) retina from Nrl^−/− cpfl1 mice. The data are presented as mean ± S.E. n = 3, *, p < 0.0017 compared with dark-adapted mice. Light-adapted retinas were obtained after mice (P30) were exposed to constant white light (71 cd/m²) in the ERG Ganzfeld for 15 min. Dark-adapted retinas were obtained from mice after overnight adaptation.