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N-Glycosylation is required for the plasma membrane targeting of PRiMA-linked mixed chicken-human AChE tetramer. HEK293T cells were transfected with different combinations of cDNAs as indicated. After 48 h, cells were immuno-stained with anti-chicken AChE or anti-human AChE antibody without or with permeabilization by Triton X-100 (0.2%) to visualize AChE on the plasma membrane or total AChE, including subcellular compartments, respectively. Bar, 20 μm.
