TABLE 1.

N-Glycosylation affects the catalytic function of AChE

Cell lysates from HEK293T cells expressing AChE_T^WT, AChE_T^N296Q, AChE_T^N381Q, AChE_T^N495Q, or AChE_T^{N296Q/N381Q/N495Q} with (mainly G₄) or without (mainly G₁/G₂) PRiMA were subjected to Ellman assays using different concentrations of ATCh as substrate, which would not cause substrate inhibition on AChE. The reaction velocity was determined at each concentration of substrate. K_m values were determined according to a Lineweaver-Burk plot. Values are the means ± S.E. obtained from nine independent experiments (n = 9).

	−PRiMA	+PRiMA
	μm	μm
AChETWT	294 ± 82	261 ± 73
AChETN296Q	326 ± 98	330 ± 69
AChETN381Q	17,379 ± 2961	16,480 ± 3690
AChETN495Q	314 ± 71	307 ± 94
AChETN296Q/N381Q/N495Q	19,058 ± 3667	17,013 ± 2799