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a, sedimentation velocity absorption profiles at 280 nm of the DENV NS5 polymerase in buffer T5 (pH 7. 6, 10 °C). The concentration of the protein is 1.89 × 10−6 m; 40,000 rpm. b, dependence of the DENV polymerase sedimentation coefficient, s20,w, upon the protein concentration in buffer T5 (pH 7.6, 10 °C).
