FIGURE 2.

Biological properties of conditionally dimerizable CagA in cells. A, subcellular localization of CagA mutants. AGS cells were transfected with an HA-tagged CagA, CagAΔCM, or GyrB-CagAΔCM vector. Cells were fixed at 17 h after transfection and immunostained with an anti-HA antibody (green). Nuclei were stained with DAPI (blue). Confocal x-y images are shown. Scale bar, 10 μm. B, tyrosine phosphorylation of CagA mutants. AGS cells were transiently transfected with an HA-tagged CagA or GyrB-CagAΔCM vector in the presence or absence of 700 nm coumermycin. At 17 h after transfection, cells were harvested and lysed. TCLs were subjected to immunoblotting with the indicated antibodies. IP, immunoprecipitates.