FIGURE 6.

Qualitative analysis of hummingbird cells. A, AGS cells were transfected with an EGFP vector together with a CagA or GyrB-CagAΔCM vector. At 8 h after transfection, coumermycin was added to the culture, and cells were incubated an additional 9 h before analysis. The cell morphological change was observed by a fluorescence microscope (left). The longest diameter was measured by using images analyzed with the ImageJ software (right). Scale bar, 100 μm. B, the longest diameters of cells expressing CagA or GyrB-CagAΔCM, which were calculated from images presented in panel A (right), are shown. Red dots show cells that are longer than the maximum value of control cells. n = 200, *, p < 0.01, Mann-Whitney U test.