FIGURE 2.

BMP2 and BMP4 stimulate eNOS enzymatic activity in PAECs. bPAECs were stimulated with BMP2, BMP4, or VEGF for 30 min, and lysates were analyzed for eNOS activity by measuring the conversion of arginine to citrulline. l-NAME added to the enzyme activity assay was used to confirm that the conversion of arginine to citrulline was due to NOS activity. Data show the mean ± S.D. (error bars) of three individual experiments. *, p < 0.05 versus serum-starved control; †, p < 0.05 versus corresponding treatment with l-NAME.