FIGURE 5.

BMP2- and BMP4-stimulated eNOS phosphorylation is PKA-dependent. A and B, PAECs were incubated with or without the PKA inhibitor H89 (10 μm) 15 min prior to stimulation with BMP2 (A) or BMP4 (B) and assessed for eNOS phosphorylation. Western blots shown are representative of four individual experiments. C, PAECs were incubated with or without the PKA inhibitors H89 (10 μm) or mPKI (5 μm) 15 min prior to stimulation with 8-Br-cAMP, BMP2, or BMP4. After 60 min, the cells were lysed and assayed for the conversion of [³H]arginine to [³H]citrulline. Data are representative of three individual experiments. Error bars, S.D. *, p < 0.05 versus unstimulated control; †, p < 0.05 versus corresponding treatment without inhibitors.