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Defective eNOS phosphorylation in hPAECs from patients harboring BMPR2 mutations. A, Western blot analysis of hPAECs from a control patient or two patients with mutations in the BMPR2 gene. B and C, serum-starved hPAECs from two patients with mutations in the BMPR2 gene or from a non-PAH lung were stimulated for 45 min with VEGF, BMP2, or BMP4, and lysates were analyzed for eNOS phosphorylation at serine 1177. The blots shown are representative of three individual experiments.
