FIGURE 6.
Deletion of the third type-1 repeat does not improve I-II-III secretion. 293 cells were either untransfected controls or transfected to express I-II-III lacking the third type-1 repeat ± secretory ChEL. The cells were then pulse-labeled with 35S-amino acids for 30 min and chased in complete medium for 4 h. Cell lysates and collected chase media were immunoprecipitated with anti-Tg and analyzed by reducing SDS-PAGE and fluorography (a nonspecific band was recovered in the media even from untransfected control cells). The positions of molecular mass marker proteins are shown.