FIGURE 3.

Analysis of the AR transgene expression in the prostate of theR26hAR^loxP/wt:Osr1-Cre+ mice. A and B, adjacent prostate sections from 16-week old male R26hAR^loxP/wt:Osr1-Cre+(A1 and A2) or R26hAR^loxP/wt:Osr1-Cre− (B1 and B2) mice were prepared and stained with either the antibody specifically against the human AR (A1 and B1) or human and mouse AR (A2 and B2) to distinguish exogenous human AR from endogenous mouse AR. Representative images are shown. C, a prostate section isolated from a 16-week-old R26hAR^loxP/wt:Osr1-Cre+ mouse was co-stained with the antibody against the human AR protein (C1) and the antibody against both human and mouse AR proteins (C2). The merged image (C3) shows overlap of both exogenous and endogenous AR proteins (arrows). D, the antibody against the human AR protein (red, D1 and D4) and the antibody against the p63 protein (green, D2 and D5) were used to co-stain prostate tissues isolated from R26hAR^loxP/wt:Osr1-Cre+ mice. The merged images show overlay of immunostaining with two antibodies (arrows, D3 and D6). E, similar co-stain analyses were performed using prostate tissues isolated from 16-week-old male R26hAR^loxP/wt:PB-Cre+ mice, in which there is no overlay of AR and p63 co-staining.