TABLE 1.
Comparison of the acceptor specificity of HNK-1ST
Sulfotransferase activity of the recombinant HNK-1ST was investigated using Chn (10 nmol as GlcUA equivalents), Chn oligosaccharides (10 nmol as oligosaccharide), glucuronylneolactotetraosylceramide (1 nmol), and GlcUA-Gal-Gal-Xyl-SGDNG (1 nmol) as acceptors under the reaction conditions described under “Experimental Procedures.”
| Acceptor | Enzymatic activity |
|
|---|---|---|
| HNK-1ST | Mock | |
| pmol/μg of protein/h | ||
| Chn polysaccharide (GlcUA-(GalNAc-GlcUA)n-GalNAc) | 1.5 ± 0.05 | NPa |
| Chn octasaccharide (GlcUA-(GalNAc-GlcUA)3-GalNAc) | 6.9 ± 0.4 | NP |
| Chn hexasaccharide (GlcUA-(GalNAc-GlcUA)2-GalNAc) | 7.0 ± 0.6 | NP |
| Chn tetrasaccharide (GlcUA-GalNAc-GlcUA-GalNAc) | 6.3 ± 2.0 | 0.2 ± 0.05 |
| Glucuronylneolactotetraosylceramide (GlcUA-Gal-GlcNAc-Gal-Glc-ceramide) | 23.4 ± 2.4 | NDb |
| GlcUA-Gal-Gal-Xyl-SGDNG | 36.3 ± 0.3 | 0.2 ± 0.05 |
a NP, not performed.
b ND, not detected.