FIGURE 2.
Expression of Plg-RKT in catecholaminergic cells. Membrane fractions were prepared from PC12 cells, Hoxa9-ER4 monocyte progenitor cells, and Hoxa9-ER4 cells differentiated with M-CSF as described (9). Lysates were prepared from human pheochromocytoma tissue, bovine adrenomedullary chromaffin cells, and mouse hippocampus. A quantity of 40 μg of protein was loaded in each lane and Western blotted with anti-Plg-RKT mAb. No bands were detected with isotype control (not shown). In additional specificity controls with anti-Plg-RKT mAb, minimal expression of Plg-RKT was detected in membranes of progenitor Hox9-ER4 cells, but the 17.2-kDa Plg-RKT band was highly expressed in M-CSF-differentiated Hoxa9-ER4 cells as described (9).