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. 2011 Jul 27;286(38):33390–33400. doi: 10.1074/jbc.M111.246447

FIGURE 1.

FIGURE 1.

Agonist-dependent signaling to HDAC5. a, signaling pathway to HDAC5 nuclear export. G protein-coupled receptor (GPCR) activation leads to InsP3 production, which in turn causes local, nuclear Ca2+ release and CaMKII/PKD activation. These kinases then phosphorylate HDAC5 triggering its nuclear export. b, rabbit ventricular myocyte expressing GFP-HDAC5 exposed to 10 μm PE, with enlargements of the nucleus 2 (N2) region before and after a 60-min PE exposure. HDAC5 nuclear export was analyzed as a decrease of Fnuc/Fcyto normalized to the initial ratio (n = 12 for 100 nm ET1, 20 for 10 μm PE). c, myocytes were pretreated with 10 μm BisI or Gö6976 for 20–30 min before ET1 or PE exposure for 60 min. d, myocytes were pretreated with 1 μm KN93 or 2 μm 2-APB for 20–30 min prior to ET1 or PE exposure (***, p < 0.001).