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. 2011 Jul 22;286(38):33345–33357. doi: 10.1074/jbc.M111.253252

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Zebrafish mll effectively suppressed gadd45αa expression. A, whole mount in situ hybridization assays for gadd45αa expression in embryos at 10-somite stage (lateral view). Panel A1, gadd45αa expression in embryos without mll morpholino injection (control); panel A2, gadd45αa expression in embryos injected with mll-MO1 (8 ng/per embryo); panel A3, gadd45αa expression in embryos injected with mll-MO2 (8 ng/per embryo); panel A4, gadd45αa expression in embryos injected with both mll-MO1 (8 ng/per embryo) and p53-MO(8 ng/per embryo). B, promoter luciferase reporter assays for gadd45αa regulation by either mll overexpression or mll knockdown in embryos. Panel B1, gadd45αa promoter activity was suppressed by human MLL overexpression significantly (p < 0.05); panel B2, gadd45αa promoter activity was enhanced by mll knockdown significantly (p < 0.0001). C, gadd45αb expression in embryos without mll morpholino injection (control) or injected with mll-MO1 (8 ng/per embryos) at 10-somite stage (lateral view). Panel C1, gadd45αb expression in embryos without mll morpholino injection (control); panel C2, gadd45αa expression in embryos injected with mll-MO1; D, morphology of embryos without mRNA injection (control) or injected with gadd45αa mRNA (400 ng/per embryo) at 48-hpf stage. Panel D1, control embryos; panel D2, embryos injected with gadd45αa mRNA. E, expression patterns of myoD and pax2a in embryos without mRNA injection (control) or injected with gadd45αa mRNA (400 pg/per embryo) at 10-somite stage. Panels E1 and E2, myoD; panel E3 and E4, pax2a. F, expression patterns of scl, lmo2, gata1, and pu.1 in embryos without mRNA injection (control) or injected with gadd45αa mRNA (400 ng/per embryo) at 10-somite stage. Panels F1 and F2, scl; panels F3 and F4, lmo2; panels F5 and F6, gata1; panels F7 and F8, pu.1. G, gadd45αa mRNA (400 ng/per embryo) injection could partially rescue scl and gata1 expression in mll-MO1 morphants. Panels G1–G3, scl rescued; panels G4–G6, gata1 rescued.