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. 2011 Jul 27;286(38):33501–33510. doi: 10.1074/jbc.M111.227678

FIGURE 1.

FIGURE 1.

Optogenetic analysis of cholinergic signaling at the NMJ in DAPC(lf) mutants. A, representative traces of synaptic currents elicited in voltage-clamped ventral body wall muscle cells by 10 ms blue-light photostimulation of the ventral nerve cord at the NMJ of WT, SLO-1(lf), and DAPC(lf) alleles (colors correspond to genotypes in B). If N-AChR function is altered, we would anticipate a significant reduction in the maximal synaptic response amplitude relative to WT, as observed with ACR-16(lf) (green trace). B, averaged photoevoked muscle response amplitudes in SLO-1(lf) and DAPC(lf) alleles are similar to WT (mean ± S.E.; n ≥ 5 per genotype). C, representative traces of photoevoked currents elicited in voltage-clamped body-wall muscles bathed in DHβE, isolating the l-AChR component of the synaptic response (colors correspond to genotypes in D). D, averaged photoevoked muscle response amplitudes mediated by Lev-nAChRs are similar across SLO-1(lf) and DAPC(lf) alleles relative to WT (mean ± S.E.; n≥3 per genotype). E, representative normalized superimposed traces of photoevoked synaptic current responses in muscles reveals kinetic differences in the synaptic response across SLO-1(lf) and DAPC(lf) mutants relative to WT (colors correspond to genotypes in F). F, averaged ½-time decay of photoevoked currents are significantly slowed only in stn-1(lf) and slo-1(lf) alleles relative to WT (**, p < 0.01; mean ± S.E.; n ≥5 per genotype). Statistical significance was determined using a one-way ANOVA with Dunnett's post-test.