FIGURE 5.

Nrf2 is overexpressed in RCC, and plays an important role in HO-1 expression. A, representative photomicrographs show the expression of Nrf2 in human RCC and normal kidney tissues detected by immunohistochemistry. Brown color dots, expression of Nrf2. Scale bar, 100 μm. B, whole cell lysates were prepared from RPTEC, Caki-1, and 786-0 cells, and assayed for Nrf2 expression by Western blot analysis. C, 786-0 cells were transfected with either different concentrations (25 and 50 nm) of Nrf2 siRNA or control siRNA. Following 72 h of siRNA transfection, cells were lysed, and expression of HO-1 and Nrf2 was analyzed by Western blot analysis. β-actin was used as loading control. Results (A–C) are representative of three independent experiments.