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. 2011 Aug 2;286(38):33613–33621. doi: 10.1074/jbc.M111.245126

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — KLHL7 forms a Cul3-based complex. A, HCT116 cells transfected with FLAG-KLHL7 or mock vectors were immunoprecipitated (IP) with anti-FLAG antibody and immunoblotted with antibodies to Cul3, Roc1, and FLAG as indicated. B, schematic representations of KLHL7 (wild-type (WT) and deletion mutants). C, deletion mutants of FLAG-tagged KLHL7 were expressed in HCT116 cells, immunoprecipitated with anti-FLAG antibody, and immunoblotted with anti-Cul3 and anti-FLAG antibodies. Nonspecific bands are indicated with an asterisk. D, HeLa cells cultured on coverslips were transfected with HA-Cul3 and FLAG-KLHL7. The cells were fixed and stained with anti-HA and anti-FLAG antibodies and Hoechst 33342. Scale bar, 20 μm. E, deletion mutants of FLAG-tagged KLHL7 were co-expressed with HA-tagged full-length KLHL7 in HCT116 cells and immunoprecipitated with anti-FLAG and anti-HA antibodies. The immunoprecipitates were blotted with anti-HA and anti-FLAG antibodies.