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Ub-positive KLHL7 puncta increase with proteasome inhibition. A–C, HeLa cells cultured on coverslips were transfected with FLAG-KLHL7 and treated with either 20 μm of MG132 or DMSO and stained with antibodies to FLAG (red) and Ub (green) with anti-FK2 (A), anti-K48 (B), and anti-K63 (C). The nucleus was stained with Hoechst 33342. Scale bars, 20 μm.
