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Ub-positive KLHL7 does not increase by lysosome inhibition. A and B, HeLa cells cultured on coverslips were transfected with FLAG-KLHL7 and treated with or without NH4Cl for lysosome inhibition and stained with antibodies to FLAG and Ub with anti-FK2 (A) and anti-K48 (B). The nucleus was detected with Hoechst 33342. Scale bars, 20 μm.
