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. 2011 Jul 19;286(38):32962–32975. doi: 10.1074/jbc.M111.243899

FIGURE 4.

FIGURE 4.

Palmitoylation on cysteine 36 and 129 determines the diffusion of AKAP79 in the membrane. Diffusion analysis by FRAP of wild type and C36S,C129S ((C36,129S)) AKAP79-YFP ± MBCD treatment. A, recovery curves of fluorescence intensity presented as means ± S.E. B, confocal images of some cells after bleaching, with arrows showing spot-bleached regions. PKA cat, PKA catalytic subunit. C, simple diffusion analysis used to calculate diffusion coefficients and mobile fractions for wild type and C36S,C129S AKAP79 mutant under control conditions and following 30 min of treatment with 100 μm MBCD. D, anomalous subdiffusion analysis of FRAP data to calculate transport coefficients and α values. Data represent means ± S.E. from at least 14 cells per condition, ***, p < 0.001; **, p < 0.01; *, p < 0.05; n.s., not significant.