FIGURE 5.

Effect of inositol lipid depletion on plasma membrane localization of AKAP79. COS-7 cells were transfected with Lyn₁₁-FRB-CFP and mRFP-FKBP-Sac/INPP5E domains and the indicated AKAP79-YFP construct. Images are shown before and 2 min after the addition of 1 μm rapamycin. A, recruitment of the mRFP-FKBP-Sac/INPP5E fusion proteins upon rapamycin addition. B and C, example images of wild type or C36S,C129S mutant AKAP79-YFP constructs, respectively, co-transfected with the indicated lipid phosphatase activity. Bar charts show the mean ratio of PM/cytosolic intensities before and after rapamycin (Rap) addition (n values range from 9 to 14; statistics are the result of a two-way analysis of variance with *, p < 0.05; **, p < 0.01 and ***, p < 0.001).