FIGURE 7.

Effect of various epitope-mapped anti-uPAR monoclonal antibodies on lamellipodia formation. The inhibitory effect of various monoclonal antibodies on uPAR-induced lamellipodia is shown as bar diagrams. In panel A these mAbs were added at a final concentration of 15 μg/ml (∼100 nm) to uPAR^wt-transfected HEK293 cells already firmly adherent to the vitronectin-coated coverslips. Protrusions were scored 24 h after the addition of the respective mAbs. Identical results were obtained if these mAbs were added during cell seeding (not shown). In panel B, uPAR^W32A-transfected HEK293 cells were primed to adhere to the vitronectin-coated coverslips by seeding in the presence of 20 nm GFD for 24 h. mAbs representing the different epitope bins (R21, mR1, and R2) were added at a final concentration of 15 μg/ml (∼100 nm) in the presence of 20 nm GFD, and incubation proceeded for an additional 24 h before protrusions were scored. Each bar represents the mean value of four independent measurements. The micrographs show representative appearances of the evaluated HEK293 cells.