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GC formation was observed in the LNs, but not in the spleens, of infected mice. Frozen sections from spleen tissue (top row) and an inguinal lymph node (bottom row) from mock-infected and day 16-infected C57BL/6 mice were stained with Abs specific for B220 (red), Thy1.2 (blue), and PNA (green). Colocalization of the PNA-positive cells with the B and T cells was detected in the LNs, but not in the spleens, of infected mice. Original magnification ×200.
